of quality statisticsgnuplot < '$gnuplot_commands' 2>&1 || echo "Error running gnuplot." >&2gnuplot
set output '$output_file'
set term png size ${graph_size}
set boxwidth 0.8
set key right tmargin
set xlabel "${xlabel}"
set ylabel "${ylabel}"
set title "${title}"
set xtics 1
set ytics 1
set grid ytics
set offsets 1, 1, 1, 1
plot '${input_file}' using ${xcol}:${q1col}:${lwcol}:${rwcol}:${q3col} with candlesticks lt 1 lw 1 title 'Quartiles' whiskerbars, \
'' using ${xcol}:${medcol}:${medcol}:${medcol}:${medcol} with candlesticks lt -1 lw 2 title 'Medians'\
#if str( $use_outliers['use_outliers_type'] ) == 'use_outliers':
, "< python -c \"for xval, yvals in [ ( fields[${xcol} - 1], fields[${use_outliers['outliercol']} - 1].split( ',' ) ) for fields in [ line.rstrip( '\\n\\r' ).split( '\\t' ) for line in open( '${input_file}' ) if not line.startswith( '#' ) ] if len( fields ) > max( ${xcol} - 1, ${use_outliers['outliercol']} - 1 ) ]: print('\\n'.join( [ '%s\\t%s' % ( xval, yval ) for yval in yvals if yval ] ))\"" using 1:2 with points pt 29 title 'Outliers'
#end if
**What it does**
Creates a boxplot graph. Its main purpose is to display a distribution of quality scores produced by *NGS: QC and maniupulation -> FASTQ Summary Statistics* tool.
.. class:: warningmark
**TIP:** If you want to display a distribution of quality scores produced by *NGS: QC and maniupulation -> FASTQ Summary Statistics* and the column assignments within the tool's interface are not automatically set (they will all read "c1" in that case) set columns manually to the following values::
Column for X axis c1
Column for Q1 c7
Column for Median c8
Column for Q3 c9
Column for left whisker c11
Column for right whisker c12
Column for Outliers c13
-----
**Output Example**
* Black horizontal lines are medians
* Rectangular red boxes show the Inter-quartile Range (IQR) (top value is Q3, bottom value is Q1)
* Whiskers show outliers at max. 1.5*IQR
.. image:: ${static_path}/images/solid_qual.png
------
**Citation**
If you use this tool, please cite `Blankenberg D, Gordon A, Von Kuster G, Coraor N, Taylor J, Nekrutenko A; Galaxy Team. Manipulation of FASTQ data with Galaxy. Bioinformatics. 2010 Jul 15;26(14):1783-5. <http://www.ncbi.nlm.nih.gov/pubmed/20562416>`_
10.1093/bioinformatics/btq281