#!/usr/bin/env python """ Performs various conversions around Sanger FASTQ data usage: %prog [options] -c, --command=c: Command to run -i, --input=i: Input file to be converted -o, --outputFastqsanger=o: FASTQ Sanger converted output file for sol2std -s, --outputFastqsolexa=s: FASTQ Solexa converted output file -f, --outputFasta=f: FASTA converted output file usage: %prog command input_file output_file """ import os import sys from bx.cookbook import doc_optparse def stop_err(msg): sys.stderr.write("%s\n" % msg) sys.exit() def __main__(): # Parse Command Line options, args = doc_optparse.parse(__doc__) cmd = "fq_all2std.pl %s %s > %s" if options.command == "sol2std": cmd = cmd % (options.command, options.input, options.outputFastqsanger) elif options.command == "std2sol": cmd = cmd % (options.command, options.input, options.outputFastqsolexa) elif options.command == "fq2fa": cmd = cmd % (options.command, options.input, options.outputFasta) try: os.system(cmd) except Exception as eq: stop_err("Error converting data format.\n" + str(eq)) if __name__ == "__main__": __main__()